Analysis of scRNA-seq data (01) Introduction to single-cell RNA-seq

Single-cell RNA-seq

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Why single-cell RNA-seq

Across human tissues there is an incredible diversity of cell types, states, and interactions. To better understand these tissues and the cell types present, single-cell RNA-seq (scRNA-seq) offers a glimpse into what genes are being expressed at the level of individual cells.

Image credit: courtesy of Dr. Ayshwarya Subramanian

This exciting and cutting-edge method can be used to:

• explore which cell types are present in a tissue
• identify unknown/rare cell types or states
• elucidate the changes in gene expression during differentiation processes or across time or states
• identify genes that are differentially expressed in particular cell types between conditions (e.g. treatment or disease)
• explore changes in expression among a cell type while incorporating spatial, regulatory, and/or protein information

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